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Basic concepts of molecular pathology and clinical diagnostics including nucleic acids, DNA replication, transcription, proteins, mutations and chromosome changes that underlie inherited and acquired/infectious disease, inheritance patterns and genetics as applied to oncology, cardiac disease and organ transplants. Covers emerging molecular/ cytologic/histologic methods (amplification, hybridization and micoarrays) to detect disease markers, monitor therapy and assess identity; pharmacogenomics and legal/ethical issues of genetic testing. Prerequisites: permission of instructor.

Course includes hands-on experience with or discussion of diagnostics instrumentation and assays using nucleic acid and protein extraction, gel electrophoresis, hybridization techniques, standard and real time polymerase chain reaction PCR), reverse transcription, DNA sequencing, autoradiography, flow cytometry, microarrays and proteomics-based methods. Pre- or corequisite: MDTS 500 or permission of instructor.

Course will cover advanced techniques and technologies in molecular diagnostics. Students will gain hands on experience in diagnostic methods using relative quantification of qRT-PCR, sequencing, and fragment analysis using capillary electrophoresis. Alternative techniques and emerging technologies such as next-generation sequencing will also be discussed. Prerequisites: MDTS 500, MDTS 501 or permission of instructor. Pre- or corequisite: MDTS 600.